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Removal of the protease induced fragments. (A) Separation of the protease induced fragments by Nuvia™ HR‐S. Wash buffer, 50 mM Tris–HCl, 40 mM NaCl pH 7.4; elution buffer, 50 mM NaAc, 310 mM NaCl. (B) MS ion chromatograms for the Nuvia™ HR‐S purification fractions.
