Fig. 2. Protection in NbM pups is maternal B cell dependent but not antibody dependent.

(A) Nb-specific IgG1 levels in breast milk taken from the stomach of pups nursed by NvM or NbM. (B) Intestinal worm burdens at day 5 PI of NvM offspring infected at 14 days old with 250 × Nb L3 following intravenous administration of 150 μl of heat-inactivated serum from either NvM or NbM. (C) Intestinal worm burdens and Nb-specific serum IgG1 levels at day 5 PI of NvM offspring infected at 14 days old with 250 × Nb L3 after being fostered on either WT and IgMi NbM mice or WT and IgMi maternal Nb-specific serum IgG1 levels. (D) Three-day-old NvM pups were fostered on WT NvM/NbM or μMT NvM/NbM dams. Pups were infected when 14 days old with 250 × Nb L3 and euthanized at day 5 PI. Offspring intestinal worm burden was analyzed, and MST IL-13⁺CD3⁺CD4⁺ T cells were quantified at day 5 PI. (E) Intestinal worm burdens at day 5 PI of NvM offspring infected at 14 days old with 250 × Nb L3 after being fostered on either WT or B cell–IL-4Rα–deficient (MB1^creIL-4Rα^−/lox) dams. All data are representative of a minimum of two experimental repeats. *P < 0.05 and **P < 0.01.