Figure 7.

BALB/c mice were intranasally immunized with LP2 alone or PBS. Two weeks after final vaccination, mice were challenged orally four times with H. pylori B0. Four weeks post challenge, levels of gastric H. pylori colonization were determined by real-time quantitative PCR (A). LP2-specific (B) or peptide-specific (C) antibody profile of mice intranasally immunized with rHpaA plus LP2. Data are expressed as mean ± S.D., n = 5. *P < 0.05. Adhesion of the H. pylori strain B0 to AGS cells alone or in combination with antiserum to rHpaA or antiserum to rHpaA plus LP2 (D). The numbers of CFU/ml were determined by real-time PCR using the TaqMan method. The data shown are representative of three independent experiments with triplicate samples. Data are expressed as mean ± S.D., n = 3. ***P < 0.001, compared with blank serum group.