Figure 2.

Precocity in Actinidia chinensis transgenic lines. (a) Ectopic overexpression of Actinidia FT gene (35S:AcFT1) gave rise to in vitro non‐viable flower development. (b) CRISPR/Cas9‐mediated mutagenesis of AcCEN4 and AcCEN (U6‐CEN4) gave rise to in vitro leaf development. (c) Early flowering in tissue culture in U6‐CEN4 lines. (d) The flower appearance is normal, with the exception of a subtending leaf‐like bract (arrowhead) and leaf‐like sepals (arrow). (e) Relative expression of Actinidia CEN4,CEN and FT genes during early in vitro growth after regeneration (S1) and in shoots of established stock plants (S2), normalized to kiwifruit ACTIN and presented as means ± SE of three biological replicates.