Figure 1.

High‐level expression of TIR1 causes auxin‐independent depletion of target protein. (a) genomically integrated TIR1‐expression cassettes of strains, PADH1–409‐TIR1, PADH1–701‐TIR1, and PZ4EV‐NTIR1 used in this study. Z4EVpr promoter is induced by β‐estradiol (β‐est) whereas Padh1 promoters are constantly active. (b) Upper: Western blot of Prp22‐AID*‐6FLAG, TIR1, and Pgk1 (as visual loading control; equal amounts of total protein were loaded in each lane) at different times (min; minutes) of β‐estradiol and/or auxin incubation; lower: estimated copies/cell of TIR1 transcripts from the corresponding culture samples. (c) quantification of western blot shown in panel B