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. 2018 Oct 19;95(1):70–79. doi: 10.1002/cyto.a.23606

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© 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fluorescence intensity and fluorescence lifetime histograms for glacial blue fluorescent microspheres. (A) 1‐D fluorescence histogram and dot plot (scatter vs. fluorescence). The side scatter and fluorescence are the amplitude of the modulated signals after Fourier transformation. The glacial blue amplitude measured with the FLFC resulted in a CV of 13% indicating optimal alignment and performance of the flow cytometer. (B) Fluorescence lifetime histogram for 2,000 events of glacial blue microspheres. The mean fluorescence lifetime is 0.98 ns with standard deviation of 0.3 ns.