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. 2019 Jun 14;10:1365. doi: 10.3389/fmicb.2019.01365

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Copyright © 2019 Robben, Witte, Schoder, Stessl, Rossmanith and Mester.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the VBNC–MIC assay by temperature-dependent time-kill curves. De novo ATP production (green bars) of VBNC L. monocytogenes was determined and compared to plate count (blue bars) of culturable L. monocytogenes after incubation at 50, 60, and 70°C over a period of 180 min; n.d., not detectable.