Table 1.
Antibody characterization
| The name of the antibody (host species, clonality, specificity) | Immunogen (species recognized) | Description (manufacturer, catalog number, RRID, control conditions) | The initial concentration and dilution |
|---|---|---|---|
| Rat anti‐mouse monoclonal anti‐CD11b‐AF488 antibodies (clone M1/70) | Macrophages marker CD11b (mouse, human) | BD Biosciences, cat# 557672, RRID: AB_396784, control: absence of binding to CD11b‐negative cell subsets (lymphocytes) as determined by FACS | 0.2 mg/ml, dilution 1:100 |
| Goat polyclonal anti‐human/rat/mouse antibodies for Iba1 | Iba1/AIF1 (C‐TERMINAL) NP_116573.1; NP_001614.3 (mouse, human, rat, pig) | BioRad, cat#AHP2024, RRID: AB_2224406, control: absence of binding to Iba‐negative cell subsets (neurons, astrocytes) as determined by immunofluorescence | 0.5 mg/ml, dilution 1:200 |
| Donkey polyclonal anti‐goat IgG antibodies conjugated with AF488 | Goat IgG (H + L) (goat) | Jackson ImmunoResearch, cat# 705‐546‐147, RRID: AB_2340430, control: antibodies have minimal cross reactivity to chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins | 1 mg/ml, dilution 1:500 |
| Mouse monoclonal AF488‐conjugated antibodies for human/mouse/rat GFAP (clone GA5) | Glial Fibrillary Acidic Protein (human, mouse, rat) | EMD Millipore, cat#mab3402X, RRID: AB_11210273, control: absence of binding to GFAP‐negative cell subsets (neurons, microglia) as determined by immunofluorescence | 1 mg/ml, dilution 1:1,000 |
| Rabbit polyclonal anti‐human/mouse/rat antibodies for β3‐tubulin | Beta III Tubulin (human, mouse, rat) | EMD Millipore, cat#ab15708A4; RRID: AB_10916541, control: absence of binding to β3‐tubulin ‐negative cell subsets (astrocytes, microglia) as determined by immunofluorescence | 1 mg/ml, dilution 1:1,000 |
| Rat monoclonal anti‐mouse MHC class II‐PE‐Cy5 (clone MS/114.15.2) | MHC Class II (I‐A/I‐E) (mouse) | eBioscience, cat# 14‐5321‐81, RRID: AB_467560, control: absence of binding to MHC class II‐negative cell subsets (T lymphocytes) as determined by FACS | 0.2 mg/ml, dilution 1:200 |
| Rat monoclonal anti‐CD45‐APC‐Cy7 (clone 30‐F11) | CD45 (mouse) | BioLegend; cat# 103115, RRID: AB_312980, control: absence of binding to CD45‐negative cells (astroglial cell line) as determined by FACS | 0.2 mg/ml, dilution 1:100 |
| Rat monoclonal anti‐F4/80‐AF488 (clone BM8) | F4/80 (mouse) | BioLegend, cat# 12312, RRID: AB_893479, control: absence of binding to F4/80‐negative cell subsets (lymphocytes) as determined by FACS | 0.5 mg/ml, dilution 1:200 |
| Rat monoclonal antibodies specific for mouse Fc receptors CD16/CD32 (clone 2.4G2) | CD16/CD32 (mouse) | BD Biosciences, cat# 553141, RRID: AB_394656, control: these are blocking antibodies | 0.5 mg/ml, dilution 1:50 |
| Rabbit polyclonal antibodies for human/mouse/rat HDLs | KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL (human, mouse, rat) | Biorbyt, cat#orb1003, RRID: AB_2737031, control: absence of non‐specific bands on the western blot | 0.5 mg/ml, dilution 1:1,000 |
| Rabbit monoclonal anti‐human/mouse/rat TSG101 antibodies | Synthetic peptide from a 1–100 amino acid sequence of human TSG101 (human, mouse, rat) | Abcam, cat#ab125011, RRID: AB_10974262, control: absence of non‐specific bands on the western blot | 1 mg/ml, dilution 1:1,000 |