Table 2.

Quantitative analysis of the expression of activation markers MHC class II and CD45 on macrophages cultured alone, directly co‐cultured with neuronal cells, or cultured with neuronal cells using transwell system^†

Neuronal cells	BMDM alone (no co‐culture)	Direct co‐culture with neuronal cells	Co‐culture with neuronal cells using a transwell system
Neuronal	42 ± 10	11 ± 3**	16 ± 3**
Cell
Line (N1E115)
Primary		9 ± 2**	10 ± 2**
Cortical
Neurons

^†Bone‐marrow‐derived macrophages (BMDM) were cultured alone (no co‐culture), directly co‐cultured with neuronal cell line N1E115 and primary cortical neurons, or co‐cultured with neuronal cells using a transwell system as for Figure 1a and the percentages of MHC class II⁺CD45⁺ double‐positive cells is shown. Mean ± S D of separate experiments is shown (n = 3).

^**The difference is statistically significant when compared to BMDM cultured alone as determined by one‐way ANOVA (F(2,10) = 24,7; p < 0.0001) followed by Bonferroni post hoc test (p < 0.01 for single comparisons between two groups).