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. 2018 Dec 3;49(3):443–453. doi: 10.1002/eji.201847647

Figure 2.

Figure 2

Predominantly CD1d‐independent TLR/DC activation of IFN‐γ production in dNKT cells. dNKT cells (NKT) were sorted from spleens of 24αβ transgenic mice, and BMDC were derived from CD1d−/− and CD1d+/− mice. NKT cells + BMDC were cultured as described. Supernatants were harvested at 24 h and IFN‐γ concentration measured by ELISA. (A) dNKT cells (for sorting strategy, see Supporting Information Fig. 3) were cultured with CD1d−/− or CD1d+/− BMDC in the presence of TLR ligands. NKT cells had been pretreated with CD1d mAb to block CD1d. Bars indicate mean and SD of triplicate cultures, and the results are from one representative experiment of five independent experiments performed. Statistics indicate differences between CD1d+/− and CD1d−/− BMDC for each TLR ligand stimulation using two‐way ANOVA with uncorrected Fisher's LSD post‐hoc test (**p<0.01). (B) dNKT cells were co‐cultured with CD40−/− or C57BL/6 BMDC in the presence of TLR ligands. The graph shows a compilation of three experiments where the values from cultures with C57BL/6 BMDC were set to 100%, and the bars indicate mean and SD of three experiments.