Table 2.
Characterization of lignin and its structural moieties in different straw maturities (MS), treated with C. subvermispora (CS) and L. edodes (LE) for 7 weeks, using quantitative 13C‐IS Py‐GC/MS
| MS1a | MS2 | MS3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Parameter | Control | CS | LE | Control | CS | LE | Control | CS | LE |
| Lignin content (% w/w DM) | 23.7 | 4.7 | 8.1 | 22.6 | 4.8 | 6.9 | 23.5 | 3.5 | 5.9 |
| Amount of lignin (g per 100 g OM) | 24.2 | 3.8 | 6.2 | 23.1 | 4.1 | 5.6 | 24.0 | 3.0 | 4.8 |
| Lignin aromatic unit (%)b | |||||||||
| H | 8.9 | 13.3 | 11.1 | 9.3 | 13.3 | 11.9 | 8.8 | 13.9 | 11.4 |
| G | 62.6 | 61.3 | 63.3 | 62.5 | 59.3 | 62.9 | 61.2 | 59.3 | 62.4 |
| S | 28.5 | 25.5 | 25.6 | 28.1 | 27.5 | 25.2 | 30.0 | 26.8 | 26.2 |
| S/G | 0.46 | 0.42 | 0.40 | 0.45 | 0.46 | 0.40 | 0.49 | 0.45 | 0.42 |
| Structural moieties (%) | |||||||||
| Unsubstituted | 4.8 | 11.4 | 7.7 | 4.7 | 12.2 | 9.3 | 5.2 | 13.7 | 10.0 |
| Methyl | 2.0 | 3.7 | 2.9 | 2.1 | 3.8 | 3.0 | 2.3 | 4.5 | 3.5 |
| Ethyl | 0.1 | 0.2 | 0.2 | 0.1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 |
| Vinyl | 29.9 | 24.8 | 28.4 | 30.5 | 23.9 | 27.3 | 30.4 | 20.3 | 26.9 |
| Cα‐oxidized | 3.2 | 20.1 | 8.4 | 3.4 | 21.3 | 10.2 | 3.2 | 26.1 | 10.6 |
| Cβ‐oxidized | 1.6 | 3.7 | 2.3 | 1.5 | 3.7 | 2.6 | 1.6 | 3.9 | 2.7 |
| Cγ‐oxidized | 57.0 | 46.8 | 52.0 | 56.2 | 46.8 | 51.1 | 55.7 | 47.8 | 49.8 |
| Miscellaneous | 1.9 | 1.9 | 2.1 | 1.9 | 1.8 | 1.8 | 2.0 | 1.7 | 2.0 |
a
MS: maturity stages around harvest (MS1: Zadoks code 83; MS2: code 87; MS3: code 91).
b
G: guaiacyl lignin subunit, H: p‐hydroxyphenyl unit, S: syringyl unit.
Values are averages of three technical replicates. No statistics were carried out on the data.