Figure 4.

The circABCB10 promoted FOXR2 expression by sponging miR‐1252. A, Predicted binding sites of miR‐1252 in the 3′‐UTR of FOXR2. B, C Luciferase assay of A549 and PC9 cells cotransfected with miR‐1252 mimics and a luciferase reporter containing wt or mutant 3′‐UTR of FOXR2 (FOXR2‐Mut). D, E, The miR‐1252 overexpression suppressed FOXR2 expression at both the mRNA and protein levels in both A549 and PC9 cells. F, G, The circABCB10 knockdown suppressed FOXR2 expression at both the mRNA and protein levels in both A549 and PC9 cells. H, FOXR2 expression was rescued by transfection with an miR‐1252 inhibitor in A549 and PC9 cells. I, J, Cell proliferation was determined by CCK8 assays in A549 and PC9 cells. K, L, Transwell assays were used to measure cell migration of A549 and PC9 cells. Each experiment was repeated at least three times; **P < 0.01 and ***P < 0.001. CCK8, Cell Counting Kit‐8; FOXR2, Forkhead box 2; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; mRNA, messenger RNA; mut, mutant; NC, negative control; 3′‐UTR, 3′‐untranslated region; wt, wild type