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In vitro co-transfection of HL-1 and MCF-7 cells. Transfection of HIF-1α-PPN leads to induction of HIF-1α target gene mRNA as assessed by semi-quantitative real-time PCR, in (A) HL-1, and (B) MCF-7. Co-transfection with a PRKCBP1 expression vector reduces HIF target gene transcript. One-way ANOVA with Bonferroni post hoc test was used for statistical analysis. n = 6. Data are expressed as mean ± SD. *P < 0.05.
