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. 2018 Nov 5;115(8):1306–1319. doi: 10.1093/cvr/cvy277

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) 2018. For permissions, please email: journals.permissions@oup.com.

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Validation of gene expression and DNA methylation by real-time PCR and MeDIP. (A) mRNA abundance of Hand-1 and HK-2 genes between hypoxia and control groups in foetal heart. (B and C) DNA methylation in the promoter region of Hand-1 (B) and HK-2 (C) genes was detected by MeDIP in the foetal heart. (D) mRNA abundance of Lcn2 and Ddit4 genes between hypoxia and control groups in adult male heart. (E, F) DNA methylation in the promoter region of Lcn2 (E) and Ddit4 (F) genes was detected by MeDIP in the adult male heart. Data are expressed as mean ± SEM. Statistical significance was calculated by Student’s t-test. *P < 0.05 vs. control group. n = 4–5.