Figure 2.

Variance components (expressed as % CV) per assay. Variance component analysis partitions the total variance of all assays associated with each sample's measurements (n = 49) into site components, kit lot, and replicate. The distribution of the components by assay are highlighted by the box and violin plots. Individual samples are joined by the light gray lines; no one sample appears to have elevated variance components across assays. The impact of Goettingen's unusual results in the BioLegend assay has been removed from the analysis, leaving the BioLegend assay with variance components comparable to the other assays. (The total and site variance components with Goettingen included are shown by the dotted lines.) Note that Roche measured their samples with the equivalent of just one kit lot; hence, that assay has no kit lot variance component.