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. 2018 Dec 8;144(8):2020–2032. doi: 10.1002/ijc.31926

Figure 2.

Figure 2

Ectopic expression of IFITM1 promotes the sphere‐forming, proliferation and migration of NSCLC cell lines in vitro. (a ‐ f) Both H1299 and H1650 cancer cell lines were infected with either control (vector) or IFITM1 over‐expression construct (IFITM1 OE). Both RNA and protein were isolated to examine the overexpression of IFITM1 by RT‐qPCR (a and d) and immunoblots (b, c, e and f). A representative image was shown (b and e) and the image intensity was analyzed by Image J and quantified (c, H1299; f, H1650). (g‐i) H1299 and H1650 cells infected with control (vector) or IFITM1‐overexpression construct (IFITM1 OE) were cultured on ultra‐low attachment plates to determine the sphere‐forming ability. Representative images are shown (g). The data are shown as mean ± SEM of three independent (h, H1299; i, H1650). (j and k) NSCLC cell lines including H1299 (j) and H1650 (k) infected with control (vector) or IFITM1‐overexpression construct (IFITM1 OE) were incubated for 96 h to determine the proliferation by MTT assay. Data shown are from three independent experiments. (l‐o) H1299 and H1650 cell lines infected with control (vector) or IFITM1‐expression construct (IFITM1 OE) were seeded in a transwell for assessing migration. Cells were incubated for 16 h, stained, and quantified. (l and n) Representative images are shown. The data are shown as mean ± SEM of three independent (m, H1299; o, H1650) (*p < 0.05, **p < 0.001). [Color figure can be viewed at wileyonlinelibrary.com]