Figure 4.

Eluates from COP materials did not upregulate inflammatory adhesion molecules in human endothelial or epithelial cells. Plots show surface expression of inflammatory adhesion molecules E‐Selectin and ICAM‐1 as well as MHC class I molecules HLA‐A/B/C. Endothelial cells (A) and epithelial cells (B) were incubated with eluates from material, medium control or TNFα for 6 hours, 24 hours, or 48 hours. Cells were gated to aquire 5000 viable cells per condition. Results are shown for COP 1 (light orange), COP 2 (orange), COP 3 (dark orange), Medium (green), and TNF (red). Unstained cells and IgG controls are shown in blue.