Figure 3.

LIMK2 overexpression in UM‐UC‐3 cells promotes proliferation and cell cycle progression, inhibits apoptosis, and affects expression of multiple cell cycle regulators. Cells were stably transfected with LIMK2‐targeted shRNA (sh‐LIMK2), scrambled control siRNA (Scramble), LIMK2 overexpression vector (LIMK2), or empty vector (Vector). (a and b) Cell proliferation measured by MTT and EdU assays. (c) Cell cycle distribution of UM‐UC‐3 cells measured by flow cytometry. (d) Cell apoptosis measured by TUNEL assays. (e) Western blotting of LIMK2, p27, cyclinD1 and ki67. β‐actin was used as a loading control.