Skip to main content
. 2019 Jun 1;26(3):e418–e421. doi: 10.3747/co.26.4381

FIGURE 4.

FIGURE 4

Evidence of elevated serum CXCL10 (interferon γ–induced protein 10) and interleukin 6. (A) Chemokine levels from the patient’s serum the day before treatment (baseline) and 21 days after the first dose of combined ipilimumab and nivolumab. Average level from healthy control patients (n = 10) are shown for reference. MIP, RANTES = macrophage inflammatory proteins; MCP = monocyte chemoattractant protein. (B) Cytokine levels from the patient’s serum the day before treatment (baseline) and 21 days after the first dose of combined ipilimumab and nivolumab. Average levels from healthy control patients (n = 10) are shown for reference. IL = interleukin; TNF = tumour necrosis factor; IFN = interferon. Serum concentrations of chemokines and cytokines were measured using the Bio-Plex Pro Human Cytokine 27-plex immunoassay (Bio-Rad Laboratories, Hercules, CA, U.S.A.) per the manufacturer’s instructions. Serum samples obtained from 10 healthy donors were also tested to establish a normal range for each cytokine. Samples were run and analyzed using the Bio-Plex Magpix Multiplex Reader (Bio-Rad Laboratories).