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. Author manuscript; available in PMC: 2019 Jun 21.
Published in final edited form as: Nat Methods. 2018 Dec;15(12):989–990. doi: 10.1038/s41592-018-0227-4

Fig. 1|. Photon-count estimation and the effects of aberrations on the photometric ratio.

Fig. 1|

a, Estimated photon count for a 45-nm-diameter bead imaged with an aberration-corrected microscope as a function of analysis area on the camera. The three curves show the counts for fitting with a fully fledged vectorial PSF model, a simplified Gaussian PSF model, and TRABI. FWHM, 214 5 nm; pixel size, 80 nm. Aperture diameter of 2 × 1.86 × FWHM used as suggested by Franke et al.1. b, The photometric ratio (Gaussian fit over TRABI value) across six bead measurements as a function of the axial position. The shaded error band indicates one s.d. Area of fit, 7×7 pixels; aperture radius, 1.86 × FWHM. c, Effect on the photometric ratio for beads single-mode aberrated with r.m.s. aberration coefficients set at half (36 mλ; top row) and full diffraction limit (72 mλ; bottom row). d, Axial estimation error for seven typical aberration uncorrected microscopes. Wrms is the mean measured wavefront error. Sim., simulation; Exp., experiment.