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. 2019 Jun 21;14(6):e0218194. doi: 10.1371/journal.pone.0218194

Fig 5. Adult macaque testicular cells cultured in SSC, PS and SM culture conditions were analyzed for expression of the germ cell marker MAGEA4 in vitro.

Fig 5

Representative micrographs show cultured cells at day 7, 14 and 21 exhibiting strong expression for spermatogonial marker MAGEA4 as shown in A, B, C (20x; without DAPI) and D, E, F (20x; with DAPI). Cells cultured for 7, 14 and 21 days showing intense expression for MAGEA4 were quantified for each culture condition. Singlets (1c), duplets (2c), triplets (3c), quadruplets (4c), quintuplets (5c), small clusters (sc) of 5–10 cells and big clusters (bc) of >10 cells, as shown by white arrowheads were quantified for each culture condition and time point.