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. 2019 Jun 21;14(6):e0218670. doi: 10.1371/journal.pone.0218670

Fig 2. RT-PCR results show elevated expression of miR-203 and miR-483 and decreased expression of the specific genes (PIAS1, RORA and SH2B1) in canine AD dogs compared to the healthy controls.

Fig 2

(A) Expression levels of miR-203 and miR-483 were elevated in the plasma of canine AD dogs by approximately 2.5-fold and 1.6-fold respectively in comparison with those of the healthy controls. The canine miR-39 was used as the internal control. (B) Expression levels of the three specific genes (PIAS1, RORA and SH2B1) were significantly downregulated in PBMCs of the canine AD dogs compared to the healthy dogs. Canine GAPDH gene was used as an internal control. Each bar is representative of a triplicate experiment for each patient (Healthy: n = 8, Atopic: n = 9). Non-parameter test; ns–Not Significant, *P < 0.05. RT-PCR results represent relative expression of AD dogs normalized to that of the health controls.