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. 2019 Jun 21;15(6):e1007796. doi: 10.1371/journal.ppat.1007796

Fig 2. Modification of EBV latent gene transcripts by m6A promotes mRNA stability.

Fig 2

(A-B) RIP using METTL14 antibody to detect the overall levels of METTL14 on viral genes in LcLs and Akata cells. Primers were designed for the indicated gene regions. (C, E) MeRIP using m6A antibody to detect overall levels of m6A on viral genes in LcLs and Akata cells with shCr or shMETTL14. Primers were designed for the indicated gene regions. (D, F) The RNA levels of METTL14 in shCr or shMETTL14 cells. (G-H) Quantification of levels of viral transcripts following treatment with actinomycin D in LcLs with shCr and sh METTL14. (I) RNA expression levels of wild-type and mutant EBNA3C. GAPDH was used as the reference control. (J) MeRIP using m6A antibody to detect the overall levels of m6A in the mutant and wild-type EBNA3C. (K) Actinomycin D was used to inhibit transcription for 6 and 12 hours and the levels of EBNA3C mRNA was determined. Experiments were independently repeated three times, and results are presented as mean±s.d. from the three experiments. “**” represents p-value <0.01; “*” represents p-value <0.05; “ns” represents no significance. UI: uninduced; IN: induced.