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. 2019 Jun 21;15(6):e1007796. doi: 10.1371/journal.ppat.1007796

Fig 3. The expression of m6A enzymes in EBV infected cells and the role of METTL14, YTHDF2 and ALKBH5 on viral gene expression.

Fig 3

(A-D) 10 million B cell, LcLs, Burkitt’s lymphoma (BL) cells BL41, Akata, EBV-positive BL41, Akata-EBV cells, 293T and 293T-BAC-EBV cells uninduced (Ul) were lysed with RIPA buffer and western blot analysis was performed with indicated antibodies. To reactivate EBV, TPA (20 ng/ml) and Butyric acid (BA, 2.5 mM) were used to treat the cells for 48 hours (IN). (E-F) 10 million SavI, SavIII, MutuI and Mutu III cells were lysed with RIPA buffer and western blot analysis was performed with indicated antibodies. To reactivate EBV, TPA (20 ng/ml) and Butyric acid (BA, 2.5 mM) were used to treat the cells for 48 hours. (G-I) 5 million LcL shCr, LcL shMETTL14 (shM14), LcL shYTHDF2 (shYF2), LcL shALKBH5 (shAH5) cells were collected, lysed and subjected to western blot with indicated antibodies. (J-L) 5 million BL41-EBV shCr, BL41-EBV shMETTL14, BL41-EBV shYTHDF2, BL41-EBV shALKBH5 cells were collected, lysed and subjected to western blot with the indicated antibodies. UI: uninduced with drugs; IN: induced with drugs.