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. Author manuscript; available in PMC: 2020 Mar 26.
Published in final edited form as: Biochemistry. 2019 Mar 7;58(12):1660–1671. doi: 10.1021/acs.biochem.9b00068

Figure 8: Dimerization of the CTD is not dependent on temperature.

Figure 8:

(A) The reaction scheme used for the CTD dimerization assay. The SUMO-CTD construct is used in this assay for easy visualization on an SDS-PAGE gel. (B) SDS-PAGE gel showing the extent of dimerization of SUMO-CTD as determined by glutaraldehyde crosslinking. The dimeric SUMO-CTD is dissociated by treatment with 0.35 % SDS, re-associated at the indicated temperature and the extent of re-association is determined by glutaraldehyde crosslinking. Glutaraldehyde crosslinking of the native SUMO-CTD before (−) and after treatment (+) with 0.35% SDS is also shown. Glutaraldehyde crosslinking of the monomeric and the dimeric SUMO-CTD proteins results in an additional band that is indicated by an asterisk. (C) The extent of SUMO-CTD dimerization at the different temperatures were quantified and plotted as a bar graph. The error bars are standard deviations from three experiments with two different protein preparations.