A. Locations along the primary sequence and channel topology of 15 LQT3 variants tested.
B. Relationship between the voltage dependence of DIII-VSD activation (V1/2 of DIII F-V) and normalized tonic block by mexiletine. The mean ± SEM is reported for groups of 3 to 4 cells. The data were fitted with a Boltzmann function and the correlation calculated. A strong correlation (R2=0.9) between these two parameters were observed when fitted with a Boltzmann function.
C. Relationship between the SSI (V1/2 of SSI) and normalized tonic block by mexiletine. The mean ± SEM is reported for groups of 3 to 4 cells. The two parameters are not well-correlated, suggesting that channel inactivation is not a good predictor of mexiletine tonic block.