Characterization of the cbr1-2 Mutant.
(A) Growth phenotypes of 2-week-old seedlings of the Col-0 wild type and cbr1-2 on 0.5× Murashige and Skoog plates. Bar = 1 cm.
(B) Growth phenotypes of 7-week-old plants of the Col-0 wild type and cbr1-2. Bar = 5 cm.
(C) Growth phenotypes of 12-week-old mature plants of the Col-0 wild type and cbr1-2. Bar = 5 cm.
(D) Quantification of total acetyl bromide lignin content in the cell walls of 14-week-old plants of the Col-0 wild type and cbr1-2.
(E) Quantification of thioacidolytic G-lignin monomer in the cell walls of 14-week-old plants of the Col-0 wild type and cbr1-2.
(F) Quantification of thioacidolytic S-lignin monomer in the cell walls of 14-week-old plants of the Col-0 wild type and cbr1-2.
Data in (D) to (F) represent means ± sd of three biological replicates of pooled stems from at least six fully mature plants per replicate. There was no significant difference in total lignin or G- or S-lignin monomer contents in cbr1-2 compared with the Col-0 wild type (two-tailed Student’s t test). CWR, cell wall residues.