Figure 11.

Characterization of CB5D and CPR Mutants.

(A) Quantification of sinapoyl ester content in 2-week-old seedlings of the indicated genotypes. The data represent means ± sd of four biological repeats. FW, fresh weight.

(B) Histochemical observation of cross sections of 7-week-old stems (1 cm from the bottom, 80 µm thick) of the indicated genotypes with Wiesner staining for total lignin (top panels) and Mӓule staining for S-lignin (bottom panels). Bars = 30 µm.

(C) Quantification of total acetyl bromide lignin content in the cell walls of the indicated genotypes.

(D) Quantification of thioacidolytic G-lignin monomer in the cell walls of the indicated genotypes.

(E) Quantification of thioacidolytic S-lignin monomer in the cell walls of the indicated genotypes.

In (C) to (E), the data represent means ± sd of three biological replicates of pooled stems from at least six fully mature plants per replicate. CWR, cell wall residues. In (A) and (C) to (E), different letters indicate significant differences (P < 0.05; one-way ANOVA, Tukey’s HSD test) between the different genotypes.