Figure 13.

Phylogenetic Relationship of Arabidopsis Cytochrome b₅ and Related Putative Homologs (of AtCB5D) across Land Plants.

Protein sequences of 6 Arabidopsis CB5 homologs and 29 putative orthologs from different species that were obtained using the AtCB5D sequence as a query via BLAST searches of nucleotide sequences in the National Center for Biotechnology Information, and/or retrieved from the PANTHER classification system database (www.pantherdb.org), were used in the analysis. The sequences were aligned with ClustalW integrated in the MEGA v.7.0 program. The evolutionary history was inferred using the neighbor-joining method. The optimal tree with the sum of branch length = 3.19379392 is shown. The percentages (>50%) of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to construct the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method, and the units represent the number of amino acid substitutions per site.