Figure 1.

The Arabidopsis scabp3 Mutant Is Tolerant to Alkali Stress.

(A) and (D) Analysis of the NaHCO₃-response phenotype in wild-type (Col-0), scabp3 mutant, and two genetic complementation line (COM1 and COM2) seedlings. Six-day-old Col-0, scabp3, COM1, and COM2 seedlings grown on MS medium were transferred to MS medium without or with 1.5 mM NaHCO₃. Photographs were taken 7 and 12 d after transfer for control seedlings (on MS medium) and NaHCO₃ treatment seedlings, respectively.

(B) and (E) Analysis of root length of new growth for seedlings in (A) and (D). Error bars represent sd (n = 20; four plates, five seedlings per plate) from measurements using the same preparation.

(C) and (F) Analysis of fresh weight for seedlings in (A) and (D). Error bars represent sd (n = 4; four plates, five seedlings on each plate are weighed together) from measurements using the same preparation.

The experiments were performed with three biological replicates with similar results. Statistical significance in (B), (C), (E), and (F) was determined by one-way ANOVA, P < 0.05. Significant differences are indicated by different lowercase letters.