Figure 2.

SCaBP3 Interacts with the C-Terminal Region of AHA2.

(A) Yeast two-hybrid analysis of the interaction between SCaBP3 and the C terminus or Central loop of AHA2. Serial decimal dilutions of yeast cells were grown on synthetic complete medium without Leu and Trp (−LW, left panel) and on synthetic complete medium without His, Leu, and Trp (−HLW, right panel). Photographs were taken after 3 to 5 d of growth on the indicated medium.

(B) Comparative yeast two-hybrid interaction analysis of the AHA2 C terminus with the members of the SCaBP/CBL family in Arabidopsis. Photographs were taken after 3 to 5 d of growth on the indicated medium.

(C) BiFC analysis of the interaction between SCaBP3 and AHA2 in N. benthamiana (SCaBP1 was used as a negative control). Pairs of split-YFP constructs were transiently coexpressed in N. benthamiana leaves, and YFP fluorescence signal was detected using Leica SP5 confocal microscopy after 48 h of infiltration. Bars = 50 μm.

The experiments were performed with three biological replicates with similar results.