Figure 1.

Allelic Variation of CsFUL1 in Cucumber.

(A) Representative fruits of the wild cucumber C. sativa var hardwickii (left) and the East Asian type cucumber ‘Chinese Long’ (right). Bar = 5 cm.

(B) Boxplots of distribution of fruit length in 150 cucumber lines harboring the CsFUL1^A or CsFUL1^C allele. Median values are shown by horizontal lines within boxes, and the range of the 25% to 75% of the data is represented by box height (P = 1.83E−17). The P-value is calculated based on two-tailed Student's t tests.

(C) The C/A SNP at 3393 in the sixth exon caused an amino acid change from Gln (Q) to Lys (K). Boxes represent exons and lines represent introns.

(D) Lys-168 (K) in the K domain of CsFUL1 and alternatively conserved Gln (Q) in that of other species (red box). Strictly conserved residues are shaded in black, and partly conserved residues are shaded in gray. At, Arabidopsis thaliana, AT5G60910; Cla, Citrullus lanatus, Cla018544; Cm, Cucumis melo, XP_008453619.1; Cs, Cucumis sativus, Csa1G039910; Nt, Nicotiana tabacum, ABF82231; Os, Oryza sativa, AGX01588; Ph, Petunia hybrida, AAF19164; Rh, Rosa hybrid cultivar, ACS74808; Sl, Solanum lycopersicum, NP_001234173 and AAP83380; Vv, Vitis vinifera, NP_001268211; Zm, Zea mays, ABW84393.

(E) and (F) Predicated three dimensional (3D) model of CsFUL1^C protein (E) and the enlarged view of the helix (boxed in [E]) where residue Gln-168 (Q168) is situated (F).