Figure 3.

Heterologous expression of CsFUL1 in Arabidopsis.

(A) to (E) Heterologous expression of CsFUL1^A and CsFUL1^C in ful-1 mutant Arabidopsis. (A) Representative silique images of 35S:CsFUL1^A/ful-1 plants. (B) Scanning electron microscopic images of siliques in ful-1 and 35S:CsFUL1^A/ful-1. Red lines indicate the replum zones. (C) RT-qPCR analysis of CsFUL1 in the 35S:CsFUL1^A/ful-1 transgenic plants. (D) Representative images of fruits of 35S:CsFUL1^C/ful-1 plants. (E) RT-qPCR analysis of CsFUL1 in 35S:CsFUL1^C/ful-1 transgenic plants. Values are means ± se (n = 3).

(F) to (K) Overexpression of CsFUL1^A and CsFUL1^C in the wild-type Arabidopsis. (F) and (G) Representative images of fruits of 35S:CsFUL1^A plants (F) and 35S:CsFUL1^C plants (G). (H) and (I) RT-qPCR analysis of CsFUL1 in the 35S:CsFUL1^A (H) and 35S:CsFUL1^C (I) background. (J) and (K) Statistical analyses of fruit length in the 35S:CsFUL1^A (J) and 35S:CsFUL1^C lines (K). Values are means ± se (n = 30 fruits). Ler, Landsberg erecta.

Three independent samples from different plants were used for gene expression. Two-tailed Student's t tests were used to compare the transgenic lines and the corresponding controls (*P < 0.05; **P < 0.01). Bar = 1 mm in (A), (D), (F), and (G) and 100 µm in (B).