Figure 7.

CsFUL1^A Downregulated CsPIN1 and CsPIN7 Transcription.

(A) Young fruits in CsFUL1^A-OX and control lines at green bud (N1, N2-1), green-yellow bud (N2-2), yellow bud (N3), and flowering (N4) stages.

(B) The auxin concentration in CsFUL1^A (dark gray) and control (light gray) fruits. Three samples from different plants were used for auxin measurement. Values are means ± se. FW, fresh weight, IAA, indole-3-acetic acid.

(C) Expression of auxin transport genes (CsPIN1, CsPIN7, and CsPID) in young fruits of transgenic A-OX-29, C-OX-5, A-RNAi-15, and C-RNAi-16 plants. Three biological replicates were performed for gene expression. Values are means ± se.

(D) Y1H showed that CsFUL1^A and CsFUL1^C bound to different CArG-box–containing motifs of CsPIN1 and CsPIN7, but not to those of CsPID. CsCUM10 was used as the negative control; +, positive interaction; –, no interaction. AD, activation domain.

(E) Quantification of GUS activities in N. benthamiana leaves after transient expression of Pro35S:CsFUL1^A and Pro35S:CsFUL1^C with ProCsPIN1:GUS and ProCsPIN7:GUS. Four independent transfection experiments were performed for each combination. Values are means ± se.

(F) ChIP-PCR showing the in vivo binding of CsFUL1^A and CsFUL1^C to the CsPIN1 and CsPIN7 promoters. The asterisk indicates significant difference between the CsFUL1^A and CsFUL1^C alleles. Three independent experiments were performed. Values are means ± se.

Significance analysis was conducted with two-tailed Student's t tests (*P < 0.05; **P < 0.01).