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. 2019 Apr 12;31(6):1289–1307. doi: 10.1105/tpc.18.00905

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Figure 8. — Protein Interactions Detected with Y2H and BiFC.

(A) Summary of interactions performed in this study. + indicates positive interaction; * represents confirmed by BiFC; − indicates no interaction; and NA indicates nonapplicable. BD and AD represented fusion of a given protein with the DNA binding and activation domain of GAL4, respectively. Gene fusion with vector pGBKT7 or pGADT7 was used as negative controls.

(B) Y2H assays showed CsFUL1^A interacts with seven proteins including CsAGL20, while CsFUL1^C does not interact with CsAGL20. No interactions were detected between CsFUL1^A and CsFUL1^C, CsFUL2, CsALC, or CsHEC3. No homodimer or heterodimer formed between CsFUL1^A and CsFUL1^C.

(C) Interaction of CsFUL1^A with CsSEP2, CsSEP4, CsAGL20, and CsARF12 by BiFC. IND-YFPC and SPT-YFPN was used as the positive control. Columns from left to right are fluorescence, differential interference contrast, and merged channels, respectively. YFPC and YFPN represent fusion of a given protein with the C terminus and N terminus of YFP in frame, respectively. Bars = 50 µm.