Figure 5.

AUY922 administration supports the maintenance of myelinated axons in sciatic nerves of C22 mice. (A) Cross-sectional views of nerve sections from Wt (top panels) and C22 (lower panels) male mice. Micron bar, 45 μm. (B) The cross-sectional area occupied by nerve fibers in a 40 μm × 40 μm square (n = 20–25 fibers per animal; n = 6–8 mice per group) was measured and graphed as shown. Graph plotted as means ± SEM; ***P < 0.001, across the treatment groups; #P < 0.05, across the genotypes; two-tailed unpaired Student’s t-test. Correlative analyses between axon and fiber diameter measurements were obtained from sciatic nerve cross-sectional areas from (C) Wt and (D) C22 groups. (E) Comparison of trendlines between the cohorts in parts C and D. Scatter plots comparing the g-ratios (axon diameter/fiber diameter) of individual fibers plotted as a function of axon diameters in nerves of (F) Wt and (G) C22 animals. (C–H) n = 950–1100 fibers per group. (H) Trendline comparisons of graphs in parts F and G.