Table 1. Primary Antibodies Used in This Studya.
dilution |
||||
---|---|---|---|---|
species | antigen | source and catalog no. | WB | IS |
rabbit | HSP70 | Stressgen; SPA-812 | 1:3000 | n/a |
rabbit | HSP70 | Abcam; ab137680 | 1:2000 | n/a |
goat | HSP27 | Santa Cruz Biotechnology, Inc.; sc-1049 | 1:1000 | n/a |
mouse | GAPDH | Encor Biotechnology, Inc.; MCA-1D4 | 1:10 000 | n/a |
rabbit | GAPDH | Encor Biotechnology, Inc.; RPCA-GAPDH | 1:8000 | n/a |
mouse | tubulin | Sigma, St Louis, MO, USA; T6199 | 1:2000 | n/a |
mouse | tubulin | Encor Biotechnology, Inc.; MCA-1B12 | 1:10 000 | n/a |
chicken | P0 | Encor Biotechnology, Inc. | 1:500 | n/a |
rat | MBP | Chemicon; MAB386 | n/a | 1:500 |
rabbit | PMP22 | Chittoor et al., 2013 | 1:1000 | 1:250 |
rabbit | laminin | Sigma; L9393 | n/a | 1:300 |
WB: Western blotting. IS: immunostaining. n/a: not applicable.