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. 2019 Jun 7;8:e45946. doi: 10.7554/eLife.45946

Figure 9. Photoreceptor synapses and retinal lamination appear unchanged in Syt1-mutant retinas.

Figure 9.

(A) TEM images of two rod synapses each from a control and RodSyt1CKO retina with pseudocoloring of presumed horizontal cell (blue) and rod bipolar cell (magenta) dendrites. Scale bars = 200 nm. (B) Images centered on the OPL of RodSyt1CKO and ConeSyt1CKO retinas co-labeled with antibodies to CaV1.4 (magenta) and CtBP2 (ribbons, green). Solid arrowheads indicate exemplar rod ribbon complexes and the open triangle points to numerous ribbon complexes within a cone terminal. Scale bars = 5 µm. (C) Measurements of ONL, OPL, and INL thickness from RodSyt1CKO, ConeSyt1CKO, and their respective control retinas. ONL: rod control: 49.6 ± 3.79 μm, n = 4; RodSyt1CKO: 48.1 ± 1.56 μm, n = 5; p=0.69; cone control: 53.5 ± 2.88 μm, n = 6; ConeSyt1CKO: 49.5 ± 3.84 μm, n = 4; p=0.42. OPL: rod control: 5.7 ± 0.26 μm, n = 4; RodSyt1CKO: 5.3 ± 0.27 μm, n = 5; p=0.34; cone control: 5.6 ± 0.10 μm, n = 5; ConeSyt1CKO: 5.5 ± 0.31 μm, n = 5; p=0.88. INL: rod control: 43.4 ± 4.42 μm, n = 4; RodSyt1CKO: 36.0 ± 1.58 μm, n = 5; p=0.13; cone control: 45.8 ± 2.64 μm, n = 7; ConeSyt1CKO: 42.4 ± 4.49 μm, n = 4; p=0.50 (t-tests). (D) Density of cone terminals per 0.01 mm2 in control and ConeSyt1CKO retinas. Control: 132.2 ± 9.81, n = 4 retinas; ConeSyt1CKO: 122.1 ± 2.59, n = 5 retinas; p=0.30 (t-test).

Figure 9—source data 1. Data for Figure 9C: Measurements of outer nuclear layer (ONL), outer plexiform layer (OPL, and inner nuclear layer (INL) thickness from RodSyt1CKO, ConeSyt1CKO, and their respective control retinas.
Data for Figure 9D. Density of cone terminals per 0.01 mm2 in control and ConeSyt1CKO retinas.
elife-45946-fig9-data1.xlsx (85.5KB, xlsx)
DOI: 10.7554/eLife.45946.011