Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (mouse) | HRGP-Cre | PMID: 15635292 | NA | |
Genetic reagent (mouse) | Syt1flox | PMID: 28511701 | NA | Syt1: MGI:99667 |
Genetic reagent (mouse) | Ai14 | Jackson Laboratories | RRID:IMSR_JAX:007914 | PMID: 20023653 |
Genetic reagent (mouse) | Rho-iCre | Jackson Laboratories |
RRID:IMSR_JAX:015850 | PMID: 15682388 |
Antibody | Rabbit anti-calbindin | Swant | CB38; RRID:AB_2721225 |
1:10,000 |
Antibody | Mouse anti-CtBP2 | BD | 612044; RRID:AB_399431 | 1:1000 |
Antibody | Goat anti-CtBP2 | Santa Cruz | sc-5966; RRID:AB_2086774 |
1:250 |
Antibody | Rabbit anti-CaV1.4 | Dr. Amy Lee, U. of Iowa | RRID:AB_2650487 | 1:1000 |
Antibody | Peanut agglutinin (PNA, FITC conjugated) | Bionexus | BN-F44 | 1:100 |
Antibody | Peanut agglutinin (PNA, Cy-5 conjugated) | Vector Laboratories | CL-1075 | 1:100 |
Antibody | Rabbit anti-PKC | Santa Cruz | sc-10800; RRID:AB_2168560 |
1:200 |
Antibody | Rabbit anti-PSD95 | Abcam | ab18258; RRID:AB_444362 |
1:500 |
Antibody | Rabbit anti-secretagogin | Biovendor | RD181120100; RRID:AB_2034060 | 1:1000 |
Antibody | Mouse anti-Syt1*Oyster550 | Synaptic Systems | 105 011C3; RRID:AB_887827 | 1:1000 |
Antibody | Donkey anti-goat secondary (Alexa Fluor 488 conjugated) |
Thermo Fisher Scientific | A11055; RRID:AB_142672 | 1:200 |
Antibody | Goat anti-mouse-FITC | BD | 554001; RRID:AB_395197 | 1:200 |
Antibody | Goat anti-rabbit secondaries (Alexa Fluor 488 and 568 conjugated) |
Thermo Fisher Scientific | A11008, A11011; RRID:AB_143165, AB_143157 | 1:200 |
Antibody | Donkey anti-rabbit secondary (Alexa Fluor 568 conjugated) |
Thermo Fisher Scientific |
A10042; RRID:AB_2534017 | 1:200 |
Antibody | Donkey anti-rabbit secondary (Alexa Fluor 647 conjugated) |
Abcam |
Ab150075; RRID:AB_2752244 |
1:200 |