Key resources table.
| Reagent type | Designation | Source | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) | HEK 293T | ATCC | ATCC Cat# CRL-3216, RRID:CVCL_0063 | |
| Transfectedconstruct (Rattus norvegicus) | SNAP-mGluR2 | Doumazane et al., 2011 | ||
| Transfected construct (Rattus norvegicus) | SNAP-mGluR2-TMD |
Doumazane et al., 2011
(modified) |
Following SNAP tag, mGluR2 truncatd to position Q558. | |
| Transfected construct (Rattus norvegicus) | SNAP-mGluR3-TMD |
Doumazane et al., 2011
(modified) |
Following SNAP tag, mGluR3 truncatd to position E567. | |
| Transfected construct (H. sapiens) | SNAP-mGluR5-TMD |
Doumazane et al., 2011
(modified) |
Following SNAP tag, mGluR5 truncatd to position V570. | |
| Transfected construct (Rattus norvegicus) | SNAP-mGluR1-TMD |
Doumazane et al., 2011
(modified) |
Following SNAP tag, mGluR1 truncatd to position V583. | |
| Transfected construct (Rattus norvegicus) | SNAP-mGluR2-R57A |
Doumazane et al., 2011
(modified) |
R at position 57 replaced with A. | |
| Transfected construct (Rattus norvegicus) | EGFP-TM-GαoA-G203T |
Lober et al., 2006
(modified) |
G at position 203 replaced with T. | |
| Transfected construct (Rattus norvegicus) | CLIP-mGluR2-TMD |
Doumazane et al., 2011
(modified) |
Following CLIP tag, mGluR2 truncatd to position Q558. | |
| Transfected construct (Rattus norvegicus) | CLIP-mGluR2 | Doumazane et al., 2011 | ||
| Transfected construct (Rattus norvegicus) | SNAP-mGluR2-TMD-N735D |
Doumazane et al., 2011
(modified) |
Following SNAP tag, mGluR2 truncatd to position Q558. N at position 735 replaced with D | |
| Transfected construct (H. sapiens) | SNAP-B2AR | modified from addgene plasmid pSNAPf-ADRβ2 | Addgene Plasmid #101123 | |
| Transfected construct (H. sapiens) | GIRK1-F137S homotetramerization mutant | |||
| Transfected construct (synthetic) | tdTomato | Addgene | Addgene Plasmid #30530 | |
| Transfected construct | Gαiq3 | Concept from Conklin et al., 1993 | Conklin et al., 1993 | |
| Transfected construct | GCaMP6f | Addgene | Addgene Plasmid #73564 | |
| Antibody | Rabbit polyclonal to HA tag (Biotin) | Abcam | ab26228, RRID:AB_449023 | 10–20 nM (1:500 dilution) |
| Chemical compound, drug | NeutrAvidin | ThermoFisher | Cat # 31000 | |
| Chemical compound, drug | mPEG | Laysan Bio | Item# BIO-PEG-SVA-5K-100MG andMPEG-SVA-5K-1g | |
| Chemical compound, drug | biotinylated mPEG | Laysan Bio | Item# BIO-PEG-SVA-5K-100MG and MPEG-SVA-5K-1g | |
| Chemical compound, drug | benzylguanine (BG)-LD555 | Scott Blanchard lab (synthesized in house) |
Synthesized in-house | |
| Chemical compound, drug | benzylguanine (BG)-LD655 | Scott Blanchard lab (synthesized in house) |
Synthesized in-house | |
| Chemical compound, drug | IGEPAL | Sigma Aldrich | I8896-50 | |
| Chemical compound, drug | DDM | Sigma Aldrich | 850520P | |
| Chemical compound, drug | SNAP Surface Alexa-546 | New England Biolabs | Cat # S9132S | |
| Chemical compound, drug | CLIP Surface Alexa-547 | New England Biolabs | Cat # S9233S | |
| Chemical compound, drug | DMEM | Thermo Fisher Scientific | Cat #11995073 | |
| Chemical compound, drug | Fetal Bovine Serum | Thermo Fisher Scientific | Cat #10437028 | |
| Chemical compound, drug | Lipofectamine 3000 Transfection Reagent | Thermo Fisher Scientific | Cat # L3000015 | |
| Chemical compound, drug | Poly-L-lysine hydrobromide | Sigma Aldrich | P2636 | |
| Chemical compound, drug | Glutamate | Sigma Aldrich | Cat # 6106-04-3 | |
| Chemical compound, drug | Xanthurenic acid | Tocris | Cat # 4120 | |
| Chemical compound, drug | LY487379 | Tocris | Cat # 3283 | |
| Chemical compound, drug | BINA | Tocris | Cat # 4048 | |
| Chemical compound, drug | Ro 64–5229 | Tocris | Cat # 2913 | |
| Chemical compound, drug | MNI 137 | Tocris | Cat # 4388 | |
| Chemical compound, drug | TASP 0433864 | Tocris | Cat # 5362 | |
| Chemical compound, drug | VU0360172 | Tocris | Cat # 4323 | |
| Chemical compound, drug | LY341459 | Tocris | Cat # 1209 | |
| Chemical compound, drug | 22:1 phosphotidylcholine | Avanti Polar Lipids | Cat #850398C | |
| Chemical compound, drug | Aminonaphthalene trisulfonic acid | Thermo Fisher | Cat # A350 | |
| Chemical compound, drug | Thalium nitrate | Sigma Aldrich | Cat # 309230 | |
| Chemical compound, drug | Gramicidin A | Sigma Aldrich | Cat # 50845 | |
| Software, algorithm | LabVIEW | http://www.ni.com/en-us/shop/labview/labview-details.html | RRID:SCR_014325 | |
| Software, algorithm | ImageJ | http://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm | GraphPad Prism | https://graphpad.com | RRID:SCR_002798 | |
| Software, algorithm | ClampX | https://www.moleculardevices.com/ | RRID:SCR_011323 | |
| Software, algorithm | Clampfit | https://www.moleculardevices.com/ | RRID:SCR_011323 | |
| Software, algorithm | Microsoft Excel | https://products.office.com/en-us/excel | RRID:SCR_016137 | |
| Software, algorithm | Origin | https://www.originlab.com/ | RRID:SCR_002815 | |
| Software, algorithm | Adobe Illustrator | https://www.adobe.com/ | RRID:SCR_010279 | |
| Software, algorithm | Olympus cellSens | www.olympus-lifescience.com/cellsens | RRID:SCR_016238 |