Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (M. musculus) |
Ba/F3-hVEGFR-3/ EpoR |
Achen et al., 2000 | Murine pro-B cells expressing a chimeric VEGFR-3, from reference lab |
|
| Cell line (M. musculus) |
Ba/F3-mVEGFR-2/ EpoR |
Stacker et al., 1999b | Murine pro-B cells expressing a chimeric VEGFR-2, from reference lab |
|
| Cell line (Sus scrofa domesticus) |
PAE-VEGFR-3- StrepIII |
Leppänen et al., 2013 | Porcine aortic endothelial cells expressing strep-tagged VEGFR-3, from reference lab |
|
| Cell line (Sus scrofa domesticus) |
PAE-VEGFR-2 -StrepIII |
Anisimov et al., 2013 | Porcine aortic endothelial cells expressing strep- tagged VEGFR-2, from reference lab |
|
| Cell line (Sus scrofa domesticus) |
PAE-VEGFR-3 | Pajusola et al., 1994 | Porcine aortic endothelial cells expressing untagged VEGFR-3, from reference lab |
|
| Cell line (Sus scrofa domesticus) |
PAE-VEGFR-2 | Waltenberger et al., 1994 | Porcine aortic endothelial cells expressing untagged VEGFR-2, from reference lab |
|
| Cell line (Homo sapiens) |
293T | ATCC | RRID:CVCL_0063 | Human embryonic kidney cells, from vendor |
| Cell line (Cricetulus griseus) |
CHO DG44 | Invitrogen | Chinese hamster ovary cells, from vendor |
|
| Cell line (Drosophila melanogaster) |
Schneider S2 cells | Invitrogen | Insect cells for protein production (Drosophila expression system), from vendor |
|
| Cell line (Spodoptera frugiperda) |
Sf9 | Invitrogen | Insect cells for protein production (FactBac system), from vendor |
|
| Transfected construct |
pMT-Ex-VEGF-C-DMH | This paper. | 1271* | Production of the cathepsin D-cleaved form of VEGF-C in S2 cells |
| Transfected construct |
pMT-hygro-BiPSP- hVEGF-C-FL |
This paper. | 751* | Production of untagged pro-VEGF-C in S2 cells |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-hVEGF-C-H6 |
This paper. | 2242* | Production of the plasmin1-cleaved form of VEGF-C (primary plasmin cleavage, between VEGF-C aa 102 and 103) |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-VEGF-C- ADAMTS3-H6 |
This paper. | 2313* | Production of the ADAMTS3 -cleaved form of VEGF-C (cleavage between aa residues 111 and 112) |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-VEGF-C- KLK3-H6 |
This paper. | 2312* | Production of the KLK3-cleaved form of VEGF-C (cleavage between aa residues 114 and 115) |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-VEGF-C- CATD-H6 |
This paper. | 2315* | Production of the cathepsin D-cleaved form of VEGF-C (cleavage between aa residues 119 and 120) |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-VEGF-C- plasmin2-H6 |
This paper. | 2314* | Production of the plasmin2-cleaved form of VEGF-C (secondary plasmin cleavage, between VEGF-C aa 127 and 128) |
| Transfected construct |
pSecTagI-IgKSP- ΔNΔC-VEGF- C(C156S)-H6 |
This paper. | 2318* | Production of the mature form of the VEGF-C-C156S mutant (primary plasmin cleavage, between VEGF-C aa 102 and 103) |
| Transfected construct |
pMX-hCCBE1-StrIII | Jeltsch et al., 2014 | 1494* | Production of full-length CCBE1 |
| Transfected construct |
psubCAG-WPRE- IgKSP-ΔNΔC-hVEGF- C-KLK3 |
This paper. | 2380* | Generation of recombinant adeno-associated virus |
| Transfected construct |
psubCAG-WPRE- IgKSP-ΔNΔC-hVEGF- C-CATD |
This paper. | 2351* | Generation of recombinant adeno-associated virus |
| Transfected construct |
psubCMV-WPRE-IgKSP -ΔNΔC-hVEGF-C- ADAMTS3 |
Anisimov et al., 2009 | Generation of recombinant adeno-associated virus (positive control) |
|
| Transfected construct |
psubCMV-WPRE | Paterna et al., 2000 | Generation of recombinant adeno-associated virus (negative control) |
|
| Transformed construct |
pFB1-melSP- hVEGF-D-FL-H6 |
This paper and Achen et al., 1998 |
229* | Generation of recombinant baculovirus (FastBac system) |
| Transformed construct |
pFB1-melSP- ΔNΔC-hVEGF-D-H6 |
This paper and Achen et al., 1998 |
118* | Generation of recombinant baculovirus (FastBac system) |
| Biological sample (H. sapiens) |
human saliva | collected from authors of this paper |
||
| Biological sample (H. sapiens) |
human seminal plasma | collected from authors of this paper |
||
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
Baluk et al., 2005 | AS no. 6 | WB (1:2000) |
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
Joukov et al., 1997 | AS 882 | WB (1:1000) IP (1:500-1:1000) |
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
Joukov et al., 1997 | AS 905 | WB (1:500) |
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
This paper. | AS 885 | WB (1:250) |
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
This paper. | AS 890 | WB (1:250) |
| Antibody | anti-VEGF-C antiserum, rabbit polyclonal |
This paper. | AS no. 3/4 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Abcam | RRID:AB_2241408 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Abcam | ab135506 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Novus/ Biotechne |
NB110-61022 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Cell Signaling Technology |
RRID:AB_2213314 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Invitrogen/ThermoFisher | RRID:AB_2547246 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Sigma-Aldrich/Merck | SAB1303101 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, rabbit polyclonal |
Sigma-Aldrich /Merck |
SAB1303607 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, goat polyclonal |
R and D Systems /Biotechne |
RRID:AB_2241406 | WB (1:1000) |
| Antibody | anti-VEGF-C antibody, mouse monoclonal |
Santa Cruz Biotechnology |
RRID:AB_11012156 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, mouse monoclonal |
Santa Cruz Biotechnology |
RRID:AB_1131232 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, mouse monoclonal |
R and D Systems /Biotechne |
RRID:AB_2213313 | WB (1:500) |
| Antibody | anti-VEGF-C antibody, mouse monoclonal |
Invitrogen /ThermoFisher |
RRID:AB_2725653 | WB (1:200) |
| Antibody | anti-VEGF-C antibody, mouse monoclonal |
Sigma-Aldrich /Merck |
SAB1306762 | WB (1:100) |
| Antibody | anti-KLK3 antibody, mouse monoclonal |
Stenman et al., 1999 | 5C7 | neutralization at 2-fold molar excess |
| Antibody | anti-VEGF-D, goat polyclonal |
R and D Systems /Biotechne |
RRID:AB_355293 | WB (1:1000) |
| Antibody | anti-phosphotyrosine antibody 4G10, mouse monoclonal |
Millipore/ Merck |
RRID:AB_309678 | WB (1:5000) |
| Antibody | anti-VEGFR-2, goat polyclonal |
R and D Systems /Biotechne |
RRID:AB_355320 | WB (1:1500) |
| Antibody | anti-VEGFR-3, mouse monoclonal |
Dumont et al., 1998 | 9D9F9 | WB (1:1000) |
| Antibody | anti-CCBE1, rabbit polyclonal |
Atlas Antibodies /Sigma-Aldrich/ Merck |
RRID:AB_10794515 | WB (1:1000) |
| Antibody | Penta·His Antibody, mouse monoclonal |
Qiagen | RRID:AB_2619735 | WB (1:1500) |
| Antibody | anti-mouse Lyve-1, rabbit polyclonal |
Karkkainen et al., 2004 | IF (1:1000) | |
| Antibody | anti-mouse CD31, rat monoclonal |
BD Biosciences | RRID:AB_393571 | IF (1:500) |
| Antibody | HRP-conjugated Strep-Tactin |
IBA | 2-1502-001 | 1:100000 |
| Antibody | Donkey anti-goat IgG |
Jackson Immuno Research |
RRID:AB_2340390 | 1:2500 |
| Antibody | Goat anti-mouse IgG |
Jackson Immuno Research |
RRID:AB_10015289 | 1:2500 |
| Antibody | Goat anti-rabbit IgG |
Jackson Immuno Research |
RRID:AB_2313567 | 1:2500 |
| Antibody | Alexa 488 donkey anti-rat |
Molecular Probes/ Thermo Fisher |
RRID:AB_2535794 | 1:500 |
| Antibody | Alexa 594 donkey anti-rabbit |
Molecular Probes/ Thermo Fisher |
RRID:AB_141637 | 1:500 |
| Recombinant protein |
KLK3 |
Wu et al., 2004; Zhang et al., 1995 |
isoform B | |
| Recombinant protein |
Cathepsin D (CATD) | R and D Systems/ Biotechne |
1014-AS | |
| Recombinant protein |
pro-VEGF-C | This paper. | 751* | untagged pro-VEGF-C |
| Recombinant protein |
ΔNΔC-VEGF-C or mature VEGF-C |
Kärpänen et al., 2006 | 792* | C-terminally histagged mature human VEGF-C (minor form) |
| Recombinant protein |
VEGF-CDMH | This paper. | 2454* | DMH form of human VEGF-C expressed in S2 cells |
| Recombinant protein |
pro-VEGF-D | Achen et al., 1998 | 229* | C-terminally histagged human pro-VEGF-D |
| Recombinant protein |
ΔNΔC-VEGF-D or mature VEGF-D |
Achen et al., 1998 | 118* | C-terminally histagged mature human VEGF-D (major form) |
| Recombinant protein |
CCBE1-StrepIII | Jeltsch et al., 2014 | 1494* | StrepIII-tagged human CCBE1 protein |
| Recombinant protein |
VEGFR-3/Fc | Jeltsch et al., 2006 | 810* | human VEGFR-3 extracellular domains 1–7 fused to IgG1Fc |
| Recombinant protein |
VEGFR-2/Fc |
Leppänen et al., 2013; Leppänen et al., 2010 |
321* | human VEGFR-2 extracellular domains 1–3 fused to IgG1Fc |
| Commercial assay or kit |
Human VEGF-C Quantikine ELISA Kit |
R and D Systems/ Biotechne |
DVEC00 | |
| Chemical compound, drug |
streptactin resin/ sepharose |
IBA | 2-1201-010 | |
| Chemical compound, drug |
Protein A-Sepharose-4B beads CL-4B (PAS) |
GE Healthcare | 17-0780-01 | |
| Chemical compound, drug |
Chelex 100 | Bio-Rad | 1421253 | |
| Chemical compound, drug |
cOmplete | Roche | 11697498001 | |
| Other | VECTASHIELD mounting medium with DAPI |
Vector Laboratories | RRID:AB_2336790 | nucleic label |
*The asterisk denotes internal lab numbering of the corresponding DNA prep.