(A) Schematic representation of the timeline of blood collection following implantation of osmotic pump, MCAO, reperfusion and MRI scan in wild-type (WT) and GluN2A-KO mice. (B) Quantitative analysis of total plasma homocysteine level in WT and hyperhomocysteinemic (HHcy) mice before (0 day) and after (3 and 5 days) implantation of pump. Values are expressed as mean ± SEM (n = 3–5 / group); (C) Representative photomicrographs of T2 maps acquired from control-WT, HHcy-WT, control-GluN2A-KO and HHcy-GluN2A-KO mice following MCAO (30 min) and reperfusion (24h). (D) Quantitative analysis of total infarct volume in WT (control and HHcy) and GluN2A-KO (control or HHcy) mice. Values are expressed as mean ± SEM (control-WT: n = 8, HHcy-WT: n = 5, control-GluN2A-KO: n = 3, HHcy-GluN2A-KO: n = 3). *p < 0.01 for WT mice day 0 vs. WT mice day 3 or 5; #p < 0.01 for GluN2A-KO mice day 0 vs. GluN2A-KO mice day 3 or 5. **p < 0.001 for control-WT vs. HHcy-WT mice.