KEY RESOURCE TABLE

Reagents or Resources	Source	Identifier
Chemicals
Beta-nicotinamide adenine dinucleotide phosphate (NADPH)	Alfa-Aesar	Cat#J60387
Dihydrofolic acid (DHF)	Sigma-Aldrich	Cat#D7006
Tetrahydrofolic acid (THF)	Sigma-Aldrich	Cat#T3125
Methotrexate	Sigma-Aldrich	Cat#A6770
Isoniazid	Sigma-Aldrich	Cat#I3377
Flavin adenine dinucleotide disodium salt hydrate (FAD)	Sigma-Aldrich	Cat#F6625
2’-deoxyuridine-5’-monophosphate disodium salt (dUMP)	Alfa-Aesar	Cat#J64627
Thymidine 5’-monophosphate disodium salt (dTMP)	Sigma-Aldrich	Cat#T7004
5,10-Methylenetetrahydrofolate (mTHF)	Toronto Research Chemicals	Cat#F680350
Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG)	Thermo Scientific	Cat#R0392
para-Aminosalicylic acid	Sigma-Aldrich	Cat#A79604
Nextera XT DNA library preparation kit	Illumina	Cat#FC-131
AMPure XP beads	Beckman-Coulter	Cat#A63880
Microbial Strains
Mycobacterium tuberculosis Erdman Strain	ATCC	Cat#35801
BL21 (DE3) competent E. Coli	New England BioLabs	Cat#C2527I
Recombinant DNA
Plasmid pET-28a(+)-MtbRv271	GenScript	N/A
Plasmid pET-24d-MtbFDTS	GenScript	N/A
Plasmid pET-41a(+)-MtbDHFR	GenScript	N/A
Plasmid pET-41a(+)-HuDHFR	GenScript	N/A
Deposited Data
Macromolecular Crystallography
Structure of Mtb DHFR bound to UCP1172	This study	PDB ID: 6DDP
Structure of Mtb DHFR bound to UCP1175	This study	PDB ID: 6DDS
Structure of Mtb DHFR bound to PAS-M	This study	PDB ID: 6DDW
Structure of Hu DHFR bound to UCP1172	This study	PDB ID: 6DE4
Structure of Mtb Rv2671 bound to UCP1063	This study	PDB ID: 6DE5
Genome Sequences (BioProject ID: PRJNA523029)
Whole Genome Sequence of Wild-type Mtb	This paper	BioSample accession: SAMN10962817
Whole Genome Sequence of PAS-R folC (E153G) mutant	This paper	BioSample accession: SAMN10962818
Whole Genome Sequence of PAS-R folC (I43T) mutant	This paper	BioSample accession: SAMN10962820
Whole Genome Sequence of PAS-R thyX mutant	This paper	BioSample accession: SAMN10962819