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AKT and ERK phosphorylation in cells from KLA patients and neonatal human lymphatic endothelial cells (LEC). Western blot analysis was performed on cell lysates (20μg total protein) from KLA cells and LEC. Blots were probed for phosphorylated-AKT (p-Ser473 and p-Thr308), total AKT, p-PRAS40 (downstream target of AKT), total PRAS40, p-ERK1/2, total ERK1/2 and actin.
