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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jan 9;134:139–152. doi: 10.1016/j.freeradbiomed.2019.01.005

Figure 1. H2O2 generation in human articular chondrocytes treated with menadione and DMNQ.

Figure 1.

The H2O2 redox sensor Orp1-roGFP bacolovirus was transduced into human articular chondrocytes. Cultures were treated with 25 μM menadione, 25 μM DMNQ, or a DMSO control. (A) Images from one representative cell for control, menadione and DMNQ treated conditions are shown in heat map format (scale bar, 5 μM). (B) Quantified data is presented as mean±SEM showing the relative mean 405:488 ratio from 10 individual cells taken from 3 independent donors. Asterisks represent significant differences comparing menadione and DMNQ treated chondrocytes to DMSO control (****p<0.0001) (ANOVA). To assess mitochondrial H2O2 generation, chondrocytes were transduced with mito-Orp1-roGFP and treated with menadione (25 μM), DMNQ (25 μM), or a DMSO control. (C) Images from one representative cell for control, menadione and DMNQ treated conditions are shown in heat map format (scale bar, 5 μM). (D) Representative data from 2 independent donors (analysing 3 independent cells per donor). Presented data is relative mean 405:488 ratios from 3 individual cells taken from 1 donor.