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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Bioorg Med Chem Lett. 2019 Jun 4;29(15):1881–1885. doi: 10.1016/j.bmcl.2019.06.003

Fig. 4.

Fig. 4.

DTBN induces stable topoisomerase IIα-DNA cleavage complexes. Reactions were allowed to reach cleavage-ligation equilibrium, diluted with reaction buffer that lacked Mg2+, and persistence was measured by assessing the loss of double-stranded breaks. DNA cleavage at time zero was set to 100%. Reactions included no compound (black) or 500 μM DTBN (blue). Error bars represent standard deviations of three independent experiments.