KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti PFTAIRE-1 (C-3) | Santa Cruz Biotechnology | Cat # sc-376366 |
| Mouse monoclonal anti Flag | Sigma Aldrich | Cat # F1804-50UG |
| Rabbit polyclonal anti Cyclin Y | Bethyl Laboratories | Cat # A302-376A |
| Rabbit polyclonal anti PCTAIRE-1 | Cell Signaling Technologies | Cat # 4852 |
| Rabbit polyclonal anti PCTAIRE-2 | Sigma Aldrich | Cat # SAB1306378 |
| Rabbit monoclonal anti CDK2 | Cell Signaling Technologies | Cat # 2546 |
| Rabbit monoclonal anti CDK9 | Cell Signaling Technologies | Cat # 2316 |
| Rabbit monoclonal anti CDK10 | Cell Signaling Technologies | Cat # 36106 |
| Rabbit polyclonal anti Cyclin K | Bethyl Laboratories | Cat # A301-939A |
| Mouse monoclonal anti CDK5 | Cell Signaling Technologies | Cat # 12134 |
| Rabbit monoclonal anti GSK3α | Cell Signaling Technologies | Cat # 4337 |
| Rabbit monoclonal anti Phospho-LRP6 S1490 | Cell Signaling Technologies | Cat # 2568 |
| Rabbit monoclonal anti LRP6 | Cell Signaling Technologies | Cat # 2560 |
| Rat monoclonal anti Pol II pSer2 | EMD Millipore | Cat # 04-1571 |
| Rabbit polyclonal anti Pol II (N-20) | Santa Cruz Biotechnology | Cat # sc-899 |
| Rabbit polyclonal anti Phospho-NPM T199 | Cell Signaling Technologies | Cat # 3451 |
| Rabbit polyclonal anti NPM | Cell Signaling Technologies | Cat # 3452 |
| Rabbit monoclonal anti Phospho-RB S608 | Cell Signaling Technologies | Cat # 8147 |
| Rabbit monoclonal anti Phospho-RB S780 | Cell Signaling Technologies | Cat # 8180 |
| Rabbit polyclonal anti Phospho-RB S795 | Cell Signaling Technologies | Cat # 9301 |
| Rabbit monoclonal anti Phospho-RB S807/811 | Cell Signaling Technologies | Cat # 8516 |
| Mouse monoclonal anti RB | Cell Signaling Technologies | Cat # 9309 |
| Rabbit monoclonal anti active β-catenin | Cell Signaling Technologies | Cat # 8814 |
| Rabbit monoclonal anti β-catenin | Cell Signaling Technologies | Cat # 9582 |
| Rabbit polyclonal anti Cyclin B1 | Cell Signaling Technologies | Cat # 4138 |
| Rabbit monoclonal anti Phospho-Histone H3 S10 | Cell Signaling Technologies | Cat # 53348 |
| Mouse monoclonal anti Histone H3 | Cell Signaling Technologies | Cat # 14269 |
| Mouse monoclonal anti Tubulin | Cell Signaling Technologies | Cat # 3873 |
| Mouse monoclonal anti Actin | Cell Signaling Technologies | Cat # 3700 |
| Bacterial and Virus Strains | ||
| NEB 10-beta | NEB | Cat # C3019I |
| NEB Stable | NEB | Cat # C3040I |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| CDK14 / Cyclin Y | Life Technologies | Cat # PV6382 |
| CDK14 / Cyclin Y | Signal Chem | Cat # P15-10G-05 |
| CDK16 / Cyclin Y | Life Technologies | Cat # PV6379 |
| CDK18 / Cyclin Y | Signal Chem | Cat # P11-10G-05 |
| RB | Signal Chem | Cat # R05-55G-50 |
| CDK2 / Cyclin A | Signal Chem | Cat # C29-18G-10 |
| CDK3 / Cyclin A | Signal Chem | Cat # C30-10G-10 |
| CellTiter Glo | Promega | Cat # G7571 |
| Thymidine | Sigma Aldrich | Cat # T1895-5G |
| Peptide: RbS608 TAADMYLSPVRSPKK |
Tufts University Dept. of Physiology | Custom synthesis |
| Peptide: RbS780 STRPPTLSPIPHIPR |
Tufts University Dept. of Physiology | Custom synthesis |
| Peptide: CDC20 KEAAGPAPSPMRAAN |
Tufts University Dept. of Physiology | Custom synthesis |
| Peptide: CCAR2 NQPLLKSPAPPLLH |
Tufts University Dept. of Physiology | Custom synthesis |
| Peptide: CENPF KIFTTPLTPSQYYSG |
Tufts University Dept. of Physiology | Custom synthesis |
| Peptide: ERF EAGGPLTPRRVSS |
Tufts University Dept. of Physiology | Custom synthesis |
| Critical Commercial Assays | ||
| LanthaScreen Eu (CDK14 / Cyclin Y) | Invitrogen | Assay ID 1839 |
| LanthaScreen Eu (CDK16 / Cyclin Y) | Invitrogen | Assay ID 1838 |
| Z’LYTE (CDK2 / Cyclin A) | Invitrogen | Assay ID 314 |
| CDK14 33P Kinase Assay | Reaction Biology Corp | |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| HCT116 | ATCC | CCL-247 |
| HEK293 | ATCC | CRL-1573 |
| MDA-MB-231 | ATCC | HTB-26 |
| HepG2 | ATCC | HB-8065 |
| PATU-8988T | DSMZ | Cat # ACC 162 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| CDK14 C218S QuikChange forward: GTGCACACTGATTTATCTCAGTACATGGAC | IDT | Custom synthesis |
| CDK14 C218S QuikChange reverse: GTCCATGTACTGAGATAAATCAGTGTGCAC | IDT | Custom synthesis |
| CDK14 QuikChange sequencing: GAAGAAGAAGGGACAC | IDT | Custom synthesis |
| sgCDK14 gRNA forward: caccgTCATGACATCATCCATACCA | IDT | Custom synthesis |
| sgCDK14 gRNA reverse: aaacTGGTATGGATGATGTCATGAc | IDT | Custom synthesis |
| Recombinant DNA | ||
| pCMV6-CDK14-myc/flag | Origene | Cat # RC224426 |
| pCMV6-CDK14 C218S-myc/flag | This paper | n/a |
| pX458 | Addgene | Cat # 48138 |
| pX458-sgCDK14 | This paper | n/a |
| pCDH-CMV-MCS- | System Biosciences | Cat # CD510B-1 |
| NanoBRET CDK2 | Promega | n/a |
| NanoBRET CDK14 | Promega | n/a |
| NanoBRET CDK15 | Promega | n/a |
| NanoBRET CDK16 | Promega | n/a |
| NanoBRET CDK17 | Promega | n/a |
| NanoBRET CDK18 | Promega | n/a |
| NanoBRET Cyclin Y (carrier DNA) | Promega | n/a |
| Software and Algorithms | ||
| Prism | GraphPad | |
| FlowJo | FlowJo, LLC | |
| ImageJ | NIH | https://imagej.nih.gov/ij/download.html |
| ZEN lite | Carl Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html |
| TIDE | Brinkman et al., 2014 | https://tide.deskgen.com/ |
| GO | Ashburner et al., 2000 | http://geneontology.org/page/go-enrichment-analysis |
| R - Bioconductor | Huber et al., 2015 | https://www.bioconductor.org/packages/release/bioc/html/limma.html |
| Other | ||
| Streptavidin-agarose beads | Thermo Fisher | Cat # 20349 |
| ProLong Gold DAPI Antifade | Cell Signaling Technologies | Cat # 8961 |
| Phalloidin CruzFluor-488 | Santa Cruz | Cat # sc-363791 |