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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2018 Dec 26;134:1–8. doi: 10.1016/j.freeradbiomed.2018.12.029

Fig. 1.

Fig. 1.

Protein carbonyl (PC) and protein-bound HNE (HNE) are measures of protein oxidation and lipid peroxidation, respectively. Samples were collected approximately 72 h following treatment. (a) PC levels in plasma were significantly elevated following Dox administration of both WT and tumor necrosis factor-alpha knockout (TNFKO) mice compared to saline-treated mice. (b) PC levels in brain were significantly elevated following Dox treatment in WT mice; however, PC levels in brain of TNFKO mice were statistically unchanged from those observed in saline-treated WT and TNFKO mice. (c) Dox administration resulted in significant elevation of protein-bound HNE in both plasma and brain of WT mice. (d) In TNFKO mice, protein-bound HNE levels were significantly elevated in plasma; however, protein-bound HNE levels in brain were not significantly different than levels observed in brains from saline-treated WT or TNFKO mice. (n=6 individually employed mice per treatment group; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, NS = not significant).