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. Author manuscript; available in PMC: 2020 Jun 20.
Published in final edited form as: Cell Chem Biol. 2019 Apr 11;26(6):885–891.e4. doi: 10.1016/j.chembiol.2019.03.003

Figure 1: AKAP95, PKA, and PDE are present in the nucleus.

Figure 1:

a) Western blot images of whole-cell, non-nuclear, and nuclear HEK293T lysates reveal localization of AKAP95, PKARIIα, and PKAcat within the nucleus. PDE4D5 is also detected in nuclear fractions, though PDE3B is not. β-tubulin antibody staining shows a lack of cytosolic protein in the nuclear fraction, and probing for CREB provides a positive control for nuclear isolation. b) Representative images showing immunofluorescence staining of endogenous protein similarly detects the candidate proteins in the nucleus. Scale bars, 10 μm (See Supplemental Figure 1 for quantification of nuclear localization of PKAcat and PKA RIIα).