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. Author manuscript; available in PMC: 2020 Jun 20.
Published in final edited form as: Mol Cell. 2019 May 1;74(6):1164–1174.e4. doi: 10.1016/j.molcel.2019.04.008

Figure 2: RPRD CID Domains Recognize Acetylated and Phosphorylated CTD Peptides.

Figure 2:

Isothermal titration calorimetry (ITC) experiments measuring in-vitro binding affinity between RPRD1A and RPRD1B CID domains and modified CTD peptides.

(A) Table of CTD-39 peptides with indicated post-translational modifications and predicted RPRD CID binding moieties.

(B) Kd values measured by ITC for RPRD1B CID and modified CTD-39 binding.

(C) Kd values measured by ITC for RPRD1A CID and modified CTD-39 binding.

(D) Representative ITC plots showing effect of K7ac on RPRD1B CID-CTD interactions for the indicated modifications

(E) Representative ITC plots showing effect of K7ac on RPRD1A CID-CTD interactions for the indicated modifications.

Data is shown as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.005 using a one-tailed T test.